“One limitation [of Cre-LoxP], the focus of this editorial, is the potential loss of fidelity of Cre recombinase expression especially in the context of modeling cancer in mice.” “It is based on ...
Mice and newts were applied to the one-step Cre-loxP organism creation technology by TAx9. In this study, the researchers succeeded in synthesizing a Cre-loxP integrated vector by placing a short DNA ...
The use of a PRG allows us to monitor Cre recombinase-mediated recombination noninvasively and repeatedly in the same animal. In addition, this approach may also allow us to track the in vivo ...
Chances are if you use mice in your research, you are intimately acquainted with the Cre/LoxP recombination system. (For the uninitiated out there, this is a system to achieve tissue- or cell ...
In traditional knockout studies, the gene is deleted altogether. However, deleting genes that are essential to embryonic development is lethal. Conditional gene knockout can be activated in an adult ...
All our multiexpression systems (currently MultiBac, MultiMam and MultiColi) rely on in vitro Cre-loxP recombination of Acceptor and Donor plasmids to assemble multiprotein expression vectors. A ...
Researchers design a Cas9 enzyme that cuts DNA only in the presence of particular drug. A series of rodent experiments showed that even with abundant food and water, personal space is essential to ...
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One-step creation of Cre-loxP organisms: Precise control and modification of gene expression
Researchers at the University of Tsukuba have developed a vector that integrates the Cre enzyme gene with the specific DNA sequence loxP, which is its target. This vector allows the creation of ...
A new editorial paper was published in Oncoscience (Volume 10) on November 14, 2023, entitled, "Be mindful of potential pitfalls when using the Cre-LoxP system in cancer research." In this new ...
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